Primer Details for NODE_240222_length_241_cov_0.916667 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGAAAAGCAACATAATTCCCAACA | TCCAACTTAGCAGGATAGTGTC | Start: 2 |
| Tm | 59.959 | 57.262 | End: 144 |
| Size | 26 | 22 | 143 |
| Primer 2 details |
|---|
| Sequence | GGACACTATCCTGCTAAGTTGGA | TTTCTTTCCTGCTTTATTATTGTCCCA | Start: 122 |
| Tm | 59.549 | 59.547 | End: 221 |
| Size | 23 | 27 | 100 |
| Primer 3 details |
|---|
| Sequence | TGAAAAGCAACATAATTCCCAACA | TCCAACTTAGCAGGATAGTGTC | Start: 4 |
| Tm | 57.833 | 57.262 | End: 144 |
| Size | 24 | 22 | 141 |
| Primer 4 details |
|---|
| Sequence | CTGAAAAGCAACATAATTCCCAACA | TCCAACTTAGCAGGATAGTGTC | Start: 3 |
| Tm | 58.778 | 57.262 | End: 144 |
| Size | 25 | 22 | 142 |
| Primer 5 details |
|---|
| Sequence | TCTGAAAAGCAACATAATTCCCAACA | TGTCCCATAGTTGGCTTAAGAA | Start: 2 |
| Tm | 59.959 | 57.08 | End: 201 |
| Size | 26 | 22 | 200 |
