Primer Details for NODE_2393_length_271_cov_1.383838 |
|---|
| (ATG)8: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAATGTGTCAAGAAAGCATCATCA | TGTCGTTAACAACAAAATCAGACT | Start: 89 |
| Tm | 59.291 | 57.328 | End: 213 |
| Size | 25 | 24 | 125 |
| Primer 2 details |
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| Sequence | AGAAGAATGTGTCAAGAAAGCATCA | TGTCGTTAACAACAAAATCAGACT | Start: 86 |
| Tm | 59.233 | 57.328 | End: 213 |
| Size | 25 | 24 | 128 |
| Primer 3 details |
|---|
| Sequence | TGTGTCAAGAAAGCATCATCAAA | TGTCGTTAACAACAAAATCAGACT | Start: 93 |
| Tm | 57.229 | 57.328 | End: 213 |
| Size | 23 | 24 | 121 |
| Primer 4 details |
|---|
| Sequence | GAATGTGTCAAGAAAGCATCATCA | TGTCGTTAACAACAAAATCAGACT | Start: 90 |
| Tm | 58.054 | 57.328 | End: 213 |
| Size | 24 | 24 | 124 |
| Primer 5 details |
|---|
| Sequence | ATGTGTCAAGAAAGCATCATCAA | TGTCGTTAACAACAAAATCAGACT | Start: 92 |
| Tm | 57.03 | 57.328 | End: 213 |
| Size | 23 | 24 | 122 |
(GAA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAATGTGTCAAGAAAGCATCATCA | TGTCGTTAACAACAAAATCAGACT | Start: 89 |
| Tm | 59.291 | 57.328 | End: 213 |
| Size | 25 | 24 | 125 |
| Primer 2 details |
|---|
| Sequence | AGAAGAATGTGTCAAGAAAGCATCA | TGTCGTTAACAACAAAATCAGACT | Start: 86 |
| Tm | 59.233 | 57.328 | End: 213 |
| Size | 25 | 24 | 128 |
| Primer 3 details |
|---|
| Sequence | TGTGTCAAGAAAGCATCATCAAA | TGTCGTTAACAACAAAATCAGACT | Start: 93 |
| Tm | 57.229 | 57.328 | End: 213 |
| Size | 23 | 24 | 121 |
| Primer 4 details |
|---|
| Sequence | GAATGTGTCAAGAAAGCATCATCA | TGTCGTTAACAACAAAATCAGACT | Start: 90 |
| Tm | 58.054 | 57.328 | End: 213 |
| Size | 24 | 24 | 124 |
| Primer 5 details |
|---|
| Sequence | ATGTGTCAAGAAAGCATCATCAA | TGTCGTTAACAACAAAATCAGACT | Start: 92 |
| Tm | 57.03 | 57.328 | End: 213 |
| Size | 23 | 24 | 122 |
