Primer Details for NODE_238284_length_238_cov_0.933333 | |||
|---|---|---|---|
| (AGC)7: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTGTTGTTTTACAAGAGCTGCA | AAGAACAAGAAGAAGCTGCTGC | Start: 73 |
| Tm | 60.083 | 59.707 | End: 172 |
| Size | 24 | 22 | 100 |
| Primer 2 details | |||
| Sequence | TGTTGTTGTTTTACAAGAGCTGC | AAGAACAAGAAGAAGCTGCTGC | Start: 73 |
| Tm | 58.58 | 59.707 | End: 172 |
| Size | 23 | 22 | 100 |
| Primer 3 details | |||
| Sequence | TTGTTGTTGTTTTACAAGAGCTGC | AGAACAAGAAGAAGCTGCTGC | Start: 72 |
| Tm | 59.138 | 59.121 | End: 171 |
| Size | 24 | 21 | 100 |
| Primer 4 details | |||
| Sequence | ACGTTCTTCTTGTTGTTGTTGATGT | AGAACAAGAAGAAGCTGCTGC | Start: 48 |
| Tm | 60.106 | 59.121 | End: 171 |
| Size | 25 | 21 | 124 |
| Primer 5 details | |||
| Sequence | CAACGTTCTTCTTGTTGTTGTTGA | AGAACAAGAAGAAGCTGCTGC | Start: 46 |
| Tm | 58.849 | 59.121 | End: 171 |
| Size | 24 | 21 | 126 |
