ssr_primer
Primer Details for NODE_236428_length_249_cov_0.869318 |
|---|
| (T)22ggggtggtttttttttatttgggggggtggttaaaaccttcaaaaaa(T)11attaaaaataaaaaaaattg(T)32ggggtggtttggggtgg(T)23ggggtgggttcgccttttgtcg(T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTGGGGTGGTTTGGGGT | GAGACGGACGGAACACAGAA | Start: 127 |
| Tm | 60.253 | 59.687 | End: 232 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | TTTTTGGGGTGGTTTGGGGT | AGACGGACGGAACACAGAAA | Start: 127 |
| Tm | 60.253 | 59.252 | End: 231 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | TTTTGGGGTGGTTTGGGGTG | GAGACGGACGGAACACAGAA | Start: 128 |
| Tm | 60.983 | 59.687 | End: 232 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | GGGTGGTTTGGGGTGGTTTT | GAGACGGACGGAACACAGAA | Start: 133 |
| Tm | 60.984 | 59.687 | End: 232 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | TTTTTGGGGTGGTTTGGGGT | AGACGGACGGAACACAGAAAA | Start: 127 |
| Tm | 60.253 | 59.863 | End: 231 |
| Size | 20 | 21 | 105 |
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