Primer Details for NODE_234311_length_251_cov_0.865169 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGTTCGGCTCATTGACCC | TCCAGAGTAAACTTACAAGTCTTGA | Start: 93 |
| Tm | 60.608 | 57.644 | End: 246 |
| Size | 20 | 25 | 154 |
| Primer 2 details |
|---|
| Sequence | CGGCTCATTGACCCACGAAT | TCCAGAGTAAACTTACAAGTCTTGA | Start: 99 |
| Tm | 60.744 | 57.644 | End: 246 |
| Size | 20 | 25 | 148 |
| Primer 3 details |
|---|
| Sequence | TTGGTTCGGCTCATTGACCC | CCAGAGTAAACTTACAAGTCTTGATGT | Start: 93 |
| Tm | 60.608 | 59.342 | End: 245 |
| Size | 20 | 27 | 153 |
| Primer 4 details |
|---|
| Sequence | GGTTCGGCTCATTGACCCA | TCCAGAGTAAACTTACAAGTCTTGA | Start: 95 |
| Tm | 60.003 | 57.644 | End: 246 |
| Size | 19 | 25 | 152 |
| Primer 5 details |
|---|
| Sequence | TGGTTCGGCTCATTGACCC | TCCAGAGTAAACTTACAAGTCTTGA | Start: 94 |
| Tm | 60.003 | 57.644 | End: 246 |
| Size | 19 | 25 | 153 |
