Primer Details for NODE_233832_length_285_cov_1.089623 | |||
|---|---|---|---|
| (A)10ggaagaaaaatgaaacgtgataactaaatataataattgtaaaaaaagaaaaaaaaatatcaagtgtgaaaatagaaaggcgcaaaagagacttgt(A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAAGGCGCAAAAGAGACT | TGAGAATATTATCGGAATCACGGT | Start: 113 |
| Tm | 57.738 | 57.659 | End: 212 |
| Size | 20 | 24 | 100 |
| Primer 2 details | |||
| Sequence | AGAAAGGCGCAAAAGAGACTTG | TGAGAATATTATCGGAATCACGGT | Start: 113 |
| Tm | 59.708 | 57.659 | End: 212 |
| Size | 22 | 24 | 100 |
| Primer 3 details | |||
| Sequence | GAAAGGCGCAAAAGAGACTTGT | TTGAGAATATTATCGGAATCACGGT | Start: 114 |
| Tm | 59.967 | 58.245 | End: 213 |
| Size | 22 | 25 | 100 |
| Primer 4 details | |||
| Sequence | AGAAAGGCGCAAAAGAGACTT | TGAGAATATTATCGGAATCACGGT | Start: 113 |
| Tm | 58.423 | 57.659 | End: 212 |
| Size | 21 | 24 | 100 |
| Primer 5 details | |||
| Sequence | AGAAAGGCGCAAAAGAGACT | TGAGAATATTATCGGAATCACGGTT | Start: 113 |
| Tm | 57.738 | 58.245 | End: 212 |
| Size | 20 | 25 | 100 |
