Primer Details for NODE_233305_length_231_cov_1.949367 | |||
|---|---|---|---|
| (GT)6: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGACTCCTAATTAAGATGTGGT | CGGGCTTCTTCAATGTGTGT | Start: 48 |
| Tm | 59.04 | 58.762 | End: 230 |
| Size | 24 | 20 | 183 |
| Primer 2 details | |||
| Sequence | CCGACTCCTAATTAAGATGTGGT | CGGGCTTCTTCAATGTGTGT | Start: 49 |
| Tm | 57.722 | 58.762 | End: 230 |
| Size | 23 | 20 | 182 |
| Primer 3 details | |||
| Sequence | TTCCGACTCCTAATTAAGATGTGGT | CGGGCTTCTTCAATGTGTGT | Start: 47 |
| Tm | 59.576 | 58.762 | End: 230 |
| Size | 25 | 20 | 184 |
| Primer 4 details | |||
| Sequence | TCCGACTCCTAATTAAGATGTGG | CGGGCTTCTTCAATGTGTGT | Start: 48 |
| Tm | 57.469 | 58.762 | End: 230 |
| Size | 23 | 20 | 183 |
| Primer 5 details | |||
| Sequence | TCCGACTCCTAATTAAGATGTGGT | GGGCTTCTTCAATGTGTGTAGC | Start: 48 |
| Tm | 59.04 | 59.838 | End: 229 |
| Size | 24 | 22 | 182 |
