Primer Details for NODE_229230_length_258_cov_1.248649 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCTGATGTGTGGAGCTGA | CCCACATCTCTTCCATTTTTGTTGT | Start: 109 |
| Tm | 60.037 | 60.222 | End: 257 |
| Size | 20 | 25 | 149 |
| Primer 2 details | |||
| Sequence | TCTGCTGATGTGTGGAGCTG | CCCACATCTCTTCCATTTTTGTTGT | Start: 108 |
| Tm | 60.037 | 60.222 | End: 257 |
| Size | 20 | 25 | 150 |
| Primer 3 details | |||
| Sequence | CTGCTGATGTGTGGAGCTGA | CCCACATCTCTTCCATTTTTGTTG | Start: 109 |
| Tm | 60.037 | 58.768 | End: 257 |
| Size | 20 | 24 | 149 |
| Primer 4 details | |||
| Sequence | TCTGCTGATGTGTGGAGCTG | CCCACATCTCTTCCATTTTTGTTG | Start: 108 |
| Tm | 60.037 | 58.768 | End: 257 |
| Size | 20 | 24 | 150 |
| Primer 5 details | |||
| Sequence | CTGCTGATGTGTGGAGCTGA | CCCACATCTCTTCCATTTTTGTT | Start: 109 |
| Tm | 60.037 | 57.527 | End: 257 |
| Size | 20 | 23 | 149 |
