Primer Details for NODE_228735_length_230_cov_0.980892 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGTTTTTCTGGTTTTTCTTGTGG | ACGAGACAACAGCAGAAACCA | Start: 16 |
| Tm | 59.649 | 60.135 | End: 177 |
| Size | 25 | 21 | 162 |
| Primer 2 details |
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| Sequence | CCTGTTTTTCTGGTTTTTCTTGTGG | CGAGACAACAGCAGAAACCAC | Start: 16 |
| Tm | 59.649 | 59.738 | End: 176 |
| Size | 25 | 21 | 161 |
| Primer 3 details |
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| Sequence | CCTGTTTTTCTGGTTTTTCTTGTGG | CGAGACAACAGCAGAAACCA | Start: 16 |
| Tm | 59.649 | 58.418 | End: 176 |
| Size | 25 | 20 | 161 |
| Primer 4 details |
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| Sequence | CCTGTTTTTCTGGTTTTTCTTGTGG | ACGAGACAACAGCAGAAACC | Start: 16 |
| Tm | 59.649 | 58.417 | End: 177 |
| Size | 25 | 20 | 162 |
| Primer 5 details |
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| Sequence | TCTGGTTTTTCTTGTGGTTTTTGT | ACGAGACAACAGCAGAAACCA | Start: 24 |
| Tm | 58.141 | 60.135 | End: 177 |
| Size | 24 | 21 | 154 |
