Primer Details for NODE_228243_length_256_cov_0.830601 |
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| (T)12ggggttttggggggg(T)13gtttttaattttattaaatttttttttg(T)31cccgttttttcccg(T)26cccgtttttttaccatccaaggtttctc(T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGTGGGTTGCCGTTTTT | AAAGAGAAACCTTGGATGGTAAAAA | Start: 29 |
| Tm | 61.437 | 57.331 | End: 212 |
| Size | 19 | 25 | 184 |
| Primer 2 details |
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| Sequence | GGGGTGGGTTGCCGTTTTT | AAAAGAGAAACCTTGGATGGTAAAA | Start: 29 |
| Tm | 61.437 | 57.331 | End: 213 |
| Size | 19 | 25 | 185 |
| Primer 3 details |
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| Sequence | GGGGTGGGTTGCCGTTTTT | AAAAAGAGAAACCTTGGATGGTAAA | Start: 29 |
| Tm | 61.437 | 57.331 | End: 214 |
| Size | 19 | 25 | 186 |
| Primer 4 details |
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| Sequence | TGTTTTTGTTACTTGGTGGGGG | AAAGAGAAACCTTGGATGGTAAAAA | Start: 10 |
| Tm | 59.233 | 57.331 | End: 212 |
| Size | 22 | 25 | 203 |
| Primer 5 details |
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| Sequence | TGTTTTTGTTACTTGGTGGGGG | AAAAGAGAAACCTTGGATGGTAAAA | Start: 10 |
| Tm | 59.233 | 57.331 | End: 213 |
| Size | 22 | 25 | 204 |
