ssr_primer
Primer Details for NODE_225697_length_250_cov_0.870056 |
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| (G)10tcatgacccccccgg(C)22tgttgcttagaaggaggcttgccccaaaatttttcggctgtctagtggccaaaggtggccaaa(G)10tgggggggggtcggctgtctagtggccaaa(G)10tcatgacccccctga(C)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTAGAAGGAGGCTTGCC | CCCCTTTGGCCACTAGACAG | Start: 69 |
| Tm | 60.035 | 60.035 | End: 172 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
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| Sequence | CTTAGAAGGAGGCTTGCCCC | CCCCTTTGGCCACTAGACAG | Start: 71 |
| Tm | 60.107 | 60.035 | End: 172 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
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| Sequence | CTGTCTAGTGGCCAAAGGGG | GGCCACTAGACAGCCGAAAA | Start: 3 |
| Tm | 60.035 | 60.321 | End: 115 |
| Size | 20 | 20 | 113 |
| Primer 4 details |
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| Sequence | TGCTTAGAAGGAGGCTTGCC | TTGTCCTCGTATTCGCAGCC | Start: 69 |
| Tm | 60.035 | 60.46 | End: 246 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
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| Sequence | CTGTCTAGTGGCCAAAGGGG | TTGTCCTCGTATTCGCAGCC | Start: 3 |
| Tm | 60.035 | 60.46 | End: 246 |
| Size | 20 | 20 | 244 |
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