Primer Details for NODE_224805_length_238_cov_0.921212 |
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| (T)12aatcaaattatatttttgcacatagtttatcaaaaacaaaggtaaaaatataatttgatt(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTTGATCAGCTTGCACACA | ACCTTTGTTTTTGATAAACTATGTGCA | Start: 88 |
| Tm | 58.977 | 58.799 | End: 190 |
| Size | 21 | 27 | 103 |
| Primer 2 details |
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| Sequence | TTGATCAGCTTGCACACATTCC | ACCTTTGTTTTTGATAAACTATGTGCA | Start: 91 |
| Tm | 59.77 | 58.799 | End: 190 |
| Size | 22 | 27 | 100 |
| Primer 3 details |
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| Sequence | TGTTTGATCAGCTTGCACACA | CCTTTGTTTTTGATAAACTATGTGCA | Start: 88 |
| Tm | 58.977 | 57.413 | End: 189 |
| Size | 21 | 26 | 102 |
| Primer 4 details |
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| Sequence | TGTTTGATCAGCTTGCACACA | ACCTTTGTTTTTGATAAACTATGTGC | Start: 88 |
| Tm | 58.977 | 57.412 | End: 190 |
| Size | 21 | 26 | 103 |
| Primer 5 details |
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| Sequence | TTGATCAGCTTGCACACATTCC | ACCTTTGTTTTTGATAAACTATGTGC | Start: 91 |
| Tm | 59.77 | 57.412 | End: 190 |
| Size | 22 | 26 | 100 |
