Primer Details for NODE_223988_length_243_cov_0.905882 | |||
|---|---|---|---|
| (ATT)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGAAAAAGAATGGAAACTCC | AAGCAGCGGTCTTAGCTAGC | Start: 32 |
| Tm | 57.151 | 60.179 | End: 133 |
| Size | 22 | 20 | 102 |
| Primer 2 details | |||
| Sequence | AACCGAAAAAGAATGGAAACTCC | AAGCAGCGGTCTTAGCTAGC | Start: 31 |
| Tm | 57.804 | 60.179 | End: 133 |
| Size | 23 | 20 | 103 |
| Primer 3 details | |||
| Sequence | AAGTCGCTAGCTAAGACCGC | CGTCGACAGTCCAAATAATAATGGT | Start: 109 |
| Tm | 59.899 | 59.478 | End: 242 |
| Size | 20 | 25 | 134 |
| Primer 4 details | |||
| Sequence | GCTAGCTAAGACCGCTGCTT | CGTCGACAGTCCAAATAATAATGGT | Start: 114 |
| Tm | 60.179 | 59.478 | End: 242 |
| Size | 20 | 25 | 129 |
| Primer 5 details | |||
| Sequence | AACCGAAAAAGAATGGAAACTCC | CAGCGGTCTTAGCTAGCGAC | Start: 31 |
| Tm | 57.804 | 60.593 | End: 130 |
| Size | 23 | 20 | 100 |
