Primer Details for NODE_2235_length_257_cov_1.326087 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTGTGTGAGTGTGTGCT | ACGACAAGCTAAATACGATATTGACA | Start: 68 |
| Tm | 60.036 | 59.024 | End: 178 |
| Size | 20 | 26 | 111 |
| Primer 2 details |
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| Sequence | CTGTGTGTGTGAGTGTGTGC | ACGACAAGCTAAATACGATATTGACA | Start: 67 |
| Tm | 59.625 | 59.024 | End: 178 |
| Size | 20 | 26 | 112 |
| Primer 3 details |
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| Sequence | GCTGTGTGTGTGAGTGTGTG | ACGACAAGCTAAATACGATATTGACA | Start: 66 |
| Tm | 59.625 | 59.024 | End: 178 |
| Size | 20 | 26 | 113 |
| Primer 4 details |
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| Sequence | TGTGTGTGTGAGTGTGTGCT | CGACAAGCTAAATACGATATTGACA | Start: 68 |
| Tm | 60.036 | 57.61 | End: 177 |
| Size | 20 | 25 | 110 |
| Primer 5 details |
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| Sequence | TGTGTGTGTGAGTGTGTGCT | ACGACAAGCTAAATACGATATTGAC | Start: 68 |
| Tm | 60.036 | 57.609 | End: 178 |
| Size | 20 | 25 | 111 |
