Primer Details for NODE_222008_length_236_cov_0.852761 |
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| (T)17aactttttttaacaatactttataatacttta(T)18aacaatactttatcaatacccacttctctta(T)24a(T)16gtttttttgtgttgg(T)12atttta(T)22gttttgtgt(G)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAATACTTTATCAATACCCACTTCTC | GGAAGACCCCACACCCCC | Start: 68 |
| Tm | 57.385 | 61.658 | End: 231 |
| Size | 27 | 18 | 164 |
| Primer 2 details |
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| Sequence | CAATACTTTATCAATACCCACTTCTCT | GGAAGACCCCACACCCCC | Start: 69 |
| Tm | 57.162 | 61.658 | End: 231 |
| Size | 27 | 18 | 163 |
| Primer 3 details |
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| Sequence | AACAATACTTTATCAATACCCACTTCT | GGAAGACCCCACACCCCC | Start: 67 |
| Tm | 57.025 | 61.658 | End: 231 |
| Size | 27 | 18 | 165 |
| Primer 4 details |
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| Sequence | 231 | 231 | Start: 231 |
| Tm | 231 | 231 | End: 231 |
| Size | 231 | 231 | 231 |
| Primer 5 details |
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| Sequence | 231 | 231 | Start: 231 |
| Tm | 231 | 231 | End: 231 |
| Size | 231 | 231 | 231 |
