Primer Details for NODE_221855_length_222_cov_2.993289 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCAGAATTTACTTAAGAGCAGTGT | GGGATGTAGGAAGAGTGACCC | Start: 38 |
| Tm | 58.329 | 59.236 | End: 178 |
| Size | 26 | 21 | 141 |
| Primer 2 details |
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| Sequence | TGTTCAATAGTTACCAGATCTCAGA | GGGATGTAGGAAGAGTGACCC | Start: 20 |
| Tm | 57.282 | 59.236 | End: 178 |
| Size | 25 | 21 | 159 |
| Primer 3 details |
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| Sequence | CTCAGAATTTACTTAAGAGCAGTGT | GGGATGTAGGAAGAGTGACCC | Start: 39 |
| Tm | 57.102 | 59.236 | End: 178 |
| Size | 25 | 21 | 140 |
| Primer 4 details |
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| Sequence | TGCTACTTTTGTTCAATAGTTACCAGA | GGGATGTAGGAAGAGTGACCC | Start: 11 |
| Tm | 59.065 | 59.236 | End: 178 |
| Size | 27 | 21 | 168 |
| Primer 5 details |
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| Sequence | CCAGATCTCAGAATTTACTTAAGAGCA | GGGATGTAGGAAGAGTGACCC | Start: 33 |
| Tm | 59.016 | 59.236 | End: 178 |
| Size | 27 | 21 | 146 |
