Primer Details for NODE_221064_length_250_cov_0.870056 | |||
|---|---|---|---|
| (T)24ccaaaactaaaattc(T)15ctttattttactttacggtttttagttgtgtttggtttgtttttc(T)35ccaaaattttccaaaa(T)24ccaaaacttcttattttcccccctcttttatg(T)16: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGTTTTTAGTTGTGTTTGGT | GGGGGAAAATAAGAAGTTTTGGAA | Start: 68 |
| Tm | 57.213 | 57.572 | End: 195 |
| Size | 22 | 24 | 128 |
| Primer 2 details | |||
| Sequence | ACGGTTTTTAGTTGTGTTTGGT | AGAAAAGTTCACCGGAATAAGAAACA | Start: 68 |
| Tm | 57.213 | 59.455 | End: 246 |
| Size | 22 | 26 | 179 |
| Primer 3 details | |||
| Sequence | ACGGTTTTTAGTTGTGTTTGGT | AGTTCACCGGAATAAGAAACAAAA | Start: 68 |
| Tm | 57.213 | 57.297 | End: 241 |
| Size | 22 | 24 | 174 |
| Primer 4 details | |||
| Sequence | ACGGTTTTTAGTTGTGTTTGGT | AAGTTCACCGGAATAAGAAACAAA | Start: 68 |
| Tm | 57.213 | 57.297 | End: 242 |
| Size | 22 | 24 | 175 |
| Primer 5 details | |||
| Sequence | ACGGTTTTTAGTTGTGTTTGGT | AAAGTTCACCGGAATAAGAAACAA | Start: 68 |
| Tm | 57.213 | 57.297 | End: 243 |
| Size | 22 | 24 | 176 |
