Primer Details for NODE_220016_length_220_cov_2.095238 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTTTCATAGGTTCCAATAGTAGGT | ACCCCCAGTAGTTTTGCCTG | Start: 29 |
| Tm | 59.613 | 59.888 | End: 196 |
| Size | 26 | 20 | 168 |
| Primer 2 details |
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| Sequence | GCTTTCATAGGTTCCAATAGTAGGT | ACCCCCAGTAGTTTTGCCTG | Start: 30 |
| Tm | 58.407 | 59.888 | End: 196 |
| Size | 25 | 20 | 167 |
| Primer 3 details |
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| Sequence | AGCTTTCATAGGTTCCAATAGTAGG | ACCCCCAGTAGTTTTGCCTG | Start: 29 |
| Tm | 58.168 | 59.888 | End: 196 |
| Size | 25 | 20 | 168 |
| Primer 4 details |
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| Sequence | AGCTTTCATAGGTTCCAATAGTAGGT | CCCAGTAGTTTTGCCTGCGA | Start: 29 |
| Tm | 59.613 | 60.605 | End: 193 |
| Size | 26 | 20 | 165 |
| Primer 5 details |
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| Sequence | GCTTTCATAGGTTCCAATAGTAGGTT | ACCCCCAGTAGTTTTGCCTG | Start: 30 |
| Tm | 58.945 | 59.888 | End: 196 |
| Size | 26 | 20 | 167 |
