Primer Details for NODE_217546_length_229_cov_0.846154 |
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| (T)16ccc(T)10cccttttattttggttttttataatgataaagggaaagagaaagatcaaattaatatatctttcaaac(T)19ccctttttttccc(T)10c(T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGATAAAGGGAAAGAGAAAGATCAAAT | AGGTGGAAATCTTGGAGAAAAAGA | Start: 53 |
| Tm | 57.241 | 58.295 | End: 226 |
| Size | 27 | 24 | 174 |
| Primer 2 details |
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| Sequence | ATGATAAAGGGAAAGAGAAAGATCAAA | AGGTGGAAATCTTGGAGAAAAAGA | Start: 52 |
| Tm | 57.241 | 58.295 | End: 226 |
| Size | 27 | 24 | 175 |
| Primer 3 details |
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| Sequence | AATGATAAAGGGAAAGAGAAAGATCAA | AGGTGGAAATCTTGGAGAAAAAGA | Start: 51 |
| Tm | 57.241 | 58.295 | End: 226 |
| Size | 27 | 24 | 176 |
| Primer 4 details |
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| Sequence | TGATAAAGGGAAAGAGAAAGATCAAAT | AGGTGGAAATCTTGGAGAAAAAGAA | Start: 53 |
| Tm | 57.241 | 58.864 | End: 226 |
| Size | 27 | 25 | 174 |
| Primer 5 details |
|---|
| Sequence | TGATAAAGGGAAAGAGAAAGATCAAAT | AAGGTGGAAATCTTGGAGAAAAAGA | Start: 53 |
| Tm | 57.241 | 58.864 | End: 227 |
| Size | 27 | 25 | 175 |
