ssr_primer
Primer Details for NODE_217032_length_225_cov_2.532895 |
|---|
| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATGGTGGAGCTGTTCACGT | CGGAAAGGCTTCAAGGGAGG | Start: 109 |
| Tm | 59.891 | 60.678 | End: 223 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
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| Sequence | AATGGTGGAGCTGTTCACGT | GGAAAGGCTTCAAGGGAGGC | Start: 109 |
| Tm | 59.891 | 60.968 | End: 222 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
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| Sequence | ACTGGCAGTGAGGTTGTTCA | CGGAAAGGCTTCAAGGGAGG | Start: 37 |
| Tm | 59.454 | 60.678 | End: 223 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | ATGGTGGAGCTGTTCACGTG | CGGAAAGGCTTCAAGGGAGG | Start: 110 |
| Tm | 60.604 | 60.678 | End: 223 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | AATGGTGGAGCTGTTCACGT | GCGGAAAGGCTTCAAGGGA | Start: 109 |
| Tm | 59.891 | 60.303 | End: 224 |
| Size | 20 | 19 | 116 |
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