ssr_primer
Primer Details for NODE_210883_length_1330_cov_224.797136 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTCTTGGGTGCTGTAAGG | TGCCTGATAAGTCAAGCGCA | Start: 275 |
| Tm | 59.963 | 60.036 | End: 506 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | TGCTCTTGGGTGCTGTAAGG | CAAGCGCAGTCCTACTAGCA | Start: 275 |
| Tm | 59.963 | 59.827 | End: 494 |
| Size | 20 | 20 | 220 |
| Primer 3 details |
|---|
| Sequence | GCTCTTGGGTGCTGTAAGGA | TGCCTGATAAGTCAAGCGCA | Start: 276 |
| Tm | 59.675 | 60.036 | End: 506 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | GGGAAGGCAGCTTCTCTTGA | AAGATGCACCAGGTCACCAG | Start: 643 |
| Tm | 59.674 | 59.963 | End: 815 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
|---|
| Sequence | AGGGAAGGCAGCTTCTCTTG | AAGATGCACCAGGTCACCAG | Start: 642 |
| Tm | 59.672 | 59.963 | End: 815 |
| Size | 20 | 20 | 174 |
(A)10tatttttatttatttttattatatgcattcatatgcattc(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTCTTGGGTGCTGTAAGG | TGCCTGATAAGTCAAGCGCA | Start: 275 |
| Tm | 59.963 | 60.036 | End: 506 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | TGCTCTTGGGTGCTGTAAGG | CAAGCGCAGTCCTACTAGCA | Start: 275 |
| Tm | 59.963 | 59.827 | End: 494 |
| Size | 20 | 20 | 220 |
| Primer 3 details |
|---|
| Sequence | GCTCTTGGGTGCTGTAAGGA | TGCCTGATAAGTCAAGCGCA | Start: 276 |
| Tm | 59.675 | 60.036 | End: 506 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | GGGAAGGCAGCTTCTCTTGA | AAGATGCACCAGGTCACCAG | Start: 643 |
| Tm | 59.674 | 59.963 | End: 815 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
|---|
| Sequence | AGGGAAGGCAGCTTCTCTTG | AAGATGCACCAGGTCACCAG | Start: 642 |
| Tm | 59.672 | 59.963 | End: 815 |
| Size | 20 | 20 | 174 |
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