ssr_primer
Primer Details for NODE_210630_length_4461_cov_54.775068 |
|---|
| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCTAGGTCTTGGGCACTG | CGTTGAGTGCGCTGTGAAAA | Start: 4194 |
| Tm | 59.963 | 59.973 | End: 4335 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
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| Sequence | ATTACGCCTCAGAGCAGCTC | CAGTGCCCAAGACCTAGCAA | Start: 4023 |
| Tm | 59.896 | 59.963 | End: 4213 |
| Size | 20 | 20 | 191 |
| Primer 3 details |
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| Sequence | ACAGCTATGCCTCATGCTCC | TAGTCAGTCCCACCAGCTGA | Start: 3000 |
| Tm | 59.892 | 59.886 | End: 3271 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
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| Sequence | AACTACTTGGCCCGTTGTGT | CATGACTTCACAAAGCGGCC | Start: 121 |
| Tm | 59.818 | 60.11 | End: 296 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
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| Sequence | GGTCGATTGATCAGTGCCCA | CCACCCATACTATGCGACGT | Start: 1273 |
| Tm | 60.108 | 59.612 | End: 1430 |
| Size | 20 | 20 | 158 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCTAGGTCTTGGGCACTG | CGTTGAGTGCGCTGTGAAAA | Start: 4194 |
| Tm | 59.963 | 59.973 | End: 4335 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
|---|
| Sequence | ATTACGCCTCAGAGCAGCTC | CAGTGCCCAAGACCTAGCAA | Start: 4023 |
| Tm | 59.896 | 59.963 | End: 4213 |
| Size | 20 | 20 | 191 |
| Primer 3 details |
|---|
| Sequence | ACAGCTATGCCTCATGCTCC | TAGTCAGTCCCACCAGCTGA | Start: 3000 |
| Tm | 59.892 | 59.886 | End: 3271 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | AACTACTTGGCCCGTTGTGT | CATGACTTCACAAAGCGGCC | Start: 121 |
| Tm | 59.818 | 60.11 | End: 296 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | GGTCGATTGATCAGTGCCCA | CCACCCATACTATGCGACGT | Start: 1273 |
| Tm | 60.108 | 59.612 | End: 1430 |
| Size | 20 | 20 | 158 |
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