Primer Details for NODE_208292_length_232_cov_1235.220126 |
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| (T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACTGAGCAGGCTGTACTTC | GGAACTCGGCAAATAAAATTATCCG | Start: 26 |
| Tm | 59.726 | 59.087 | End: 196 |
| Size | 21 | 25 | 171 |
| Primer 2 details |
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| Sequence | CACTGAGCAGGCTGTACTTCT | GGAACTCGGCAAATAAAATTATCCG | Start: 27 |
| Tm | 59.725 | 59.087 | End: 196 |
| Size | 21 | 25 | 170 |
| Primer 3 details |
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| Sequence | TCACTGAGCAGGCTGTACTT | GGAACTCGGCAAATAAAATTATCCG | Start: 26 |
| Tm | 58.658 | 59.087 | End: 196 |
| Size | 20 | 25 | 171 |
| Primer 4 details |
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| Sequence | TTCACTGAGCAGGCTGTACT | GGAACTCGGCAAATAAAATTATCCG | Start: 25 |
| Tm | 58.658 | 59.087 | End: 196 |
| Size | 20 | 25 | 172 |
| Primer 5 details |
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| Sequence | TCACTGAGCAGGCTGTACTTC | GGAACTCGGCAAATAAAATTATCCGA | Start: 26 |
| Tm | 59.726 | 60.239 | End: 196 |
| Size | 21 | 26 | 171 |
