ssr_primer
Primer Details for NODE_202830_length_1143_cov_613.063551 |
|---|
| (TC)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCCTACTCTGTGTACCCGA | CGTTTGTCTGCAGAGGGCTA | Start: 10 |
| Tm | 60.034 | 60.037 | End: 180 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | CGGGACTGCTGCACTCTAAA | AGAATTCGGTGCAGGCGTAA | Start: 540 |
| Tm | 60.037 | 60.037 | End: 728 |
| Size | 20 | 20 | 189 |
| Primer 3 details |
|---|
| Sequence | ACCCCCTACTCTGTGTACCC | CGTTTGTCTGCAGAGGGCTA | Start: 8 |
| Tm | 59.958 | 60.037 | End: 180 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | GAAGGGGTAGCATCCACACC | TGGTGGATCTCGGTGGATCT | Start: 885 |
| Tm | 60.107 | 60.032 | End: 1133 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | TTACGCCTGCACCGAATTCT | GGTGTGGATGCTACCCCTTC | Start: 709 |
| Tm | 60.037 | 60.107 | End: 904 |
| Size | 20 | 20 | 196 |
(A)10taaatgaagttaaatgaagtgagatccaccgagatccacc(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCCTACTCTGTGTACCCGA | CGTTTGTCTGCAGAGGGCTA | Start: 10 |
| Tm | 60.034 | 60.037 | End: 180 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | CGGGACTGCTGCACTCTAAA | AGAATTCGGTGCAGGCGTAA | Start: 540 |
| Tm | 60.037 | 60.037 | End: 728 |
| Size | 20 | 20 | 189 |
| Primer 3 details |
|---|
| Sequence | ACCCCCTACTCTGTGTACCC | CGTTTGTCTGCAGAGGGCTA | Start: 8 |
| Tm | 59.958 | 60.037 | End: 180 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | GAAGGGGTAGCATCCACACC | TGGTGGATCTCGGTGGATCT | Start: 885 |
| Tm | 60.107 | 60.032 | End: 1133 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | TTACGCCTGCACCGAATTCT | GGTGTGGATGCTACCCCTTC | Start: 709 |
| Tm | 60.037 | 60.107 | End: 904 |
| Size | 20 | 20 | 196 |
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