ssr_primer
Primer Details for NODE_200164_length_2790_cov_94.549503 |
|---|
| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCTTGCCATTGCTGCAAT | TGGGCTATGAGGGGTCATGA | Start: 476 |
| Tm | 60.037 | 60.03 | End: 584 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
|---|
| Sequence | TGCCTTAAACCACCTTGCCA | TGGGCTATGAGGGGTCATGA | Start: 466 |
| Tm | 60.106 | 60.03 | End: 584 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | CGCAACCCAAGTCTTCTCCT | CACACCGTCCTGAGTCACAA | Start: 143 |
| Tm | 59.965 | 59.896 | End: 262 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
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| Sequence | GCAGGAGCCATGAAGCAAAC | AGGAGAAGACTTGGGTTGCG | Start: 15 |
| Tm | 60.109 | 59.965 | End: 162 |
| Size | 20 | 20 | 148 |
| Primer 5 details |
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| Sequence | TTGTGACTCAGGACGGTGTG | TGGCAAGGTGGTTTAAGGCA | Start: 243 |
| Tm | 59.896 | 60.106 | End: 485 |
| Size | 20 | 20 | 243 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCTTGCCATTGCTGCAAT | TGGGCTATGAGGGGTCATGA | Start: 476 |
| Tm | 60.037 | 60.03 | End: 584 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
|---|
| Sequence | TGCCTTAAACCACCTTGCCA | TGGGCTATGAGGGGTCATGA | Start: 466 |
| Tm | 60.106 | 60.03 | End: 584 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | CGCAACCCAAGTCTTCTCCT | CACACCGTCCTGAGTCACAA | Start: 143 |
| Tm | 59.965 | 59.896 | End: 262 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
|---|
| Sequence | GCAGGAGCCATGAAGCAAAC | AGGAGAAGACTTGGGTTGCG | Start: 15 |
| Tm | 60.109 | 59.965 | End: 162 |
| Size | 20 | 20 | 148 |
| Primer 5 details |
|---|
| Sequence | TTGTGACTCAGGACGGTGTG | TGGCAAGGTGGTTTAAGGCA | Start: 243 |
| Tm | 59.896 | 60.106 | End: 485 |
| Size | 20 | 20 | 243 |
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