ssr_primer
Primer Details for NODE_200070_length_975_cov_290.460089 |
|---|
| (CAG)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGTCAGCAGGTGCCAGTA | CCACAGAACTGCTCTCGGTT | Start: 603 |
| Tm | 59.965 | 59.966 | End: 746 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
|---|
| Sequence | TAAGAAGCGGCGATGCAGAA | GGAGGGATCCTGTCTCCACT | Start: 151 |
| Tm | 60.108 | 60.032 | End: 285 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | TTCTAAGAAGCGGCGATGCA | GGAGGGATCCTGTCTCCACT | Start: 148 |
| Tm | 60.108 | 60.032 | End: 285 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | GGAGTTCTAAGAAGCGGCGA | GGAGGGATCCTGTCTCCACT | Start: 144 |
| Tm | 59.827 | 60.032 | End: 285 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | GCGCATCTACAGGAGCTTCA | GGAGGGATCCTGTCTCCACT | Start: 81 |
| Tm | 60.179 | 60.032 | End: 285 |
| Size | 20 | 20 | 205 |
(GAA)8gaggag(GAA)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGTCAGCAGGTGCCAGTA | CCACAGAACTGCTCTCGGTT | Start: 603 |
| Tm | 59.965 | 59.966 | End: 746 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
|---|
| Sequence | TAAGAAGCGGCGATGCAGAA | GGAGGGATCCTGTCTCCACT | Start: 151 |
| Tm | 60.108 | 60.032 | End: 285 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | TTCTAAGAAGCGGCGATGCA | GGAGGGATCCTGTCTCCACT | Start: 148 |
| Tm | 60.108 | 60.032 | End: 285 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | GGAGTTCTAAGAAGCGGCGA | GGAGGGATCCTGTCTCCACT | Start: 144 |
| Tm | 59.827 | 60.032 | End: 285 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | GCGCATCTACAGGAGCTTCA | GGAGGGATCCTGTCTCCACT | Start: 81 |
| Tm | 60.179 | 60.032 | End: 285 |
| Size | 20 | 20 | 205 |
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