ssr_primer
Primer Details for NODE_198605_length_1521_cov_196.098066 |
|---|
| (T)30: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGCAAGCTTTGTGACAGC | ATCTGTGTGGTTGCCAGCA | Start: 956 |
| Tm | 59.335 | 59.849 | End: 1171 |
| Size | 20 | 19 | 216 |
| Primer 2 details |
|---|
| Sequence | TCTGCAAGCTTTGTGACAGC | CATCTGTGTGGTTGCCAGCA | Start: 956 |
| Tm | 59.335 | 61.174 | End: 1172 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
|---|
| Sequence | TCTGCAAGCTTTGTGACAGC | ACATCTGTGTGGTTGCCAGC | Start: 956 |
| Tm | 59.335 | 61.175 | End: 1173 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
|---|
| Sequence | TGCTGGCAACCACACAGAT | TGGAGATGCTCAAGCCAACT | Start: 1153 |
| Tm | 59.849 | 59.305 | End: 1432 |
| Size | 19 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | TGCTGGCAACCACACAGATG | TGGAGATGCTCAAGCCAACT | Start: 1153 |
| Tm | 61.174 | 59.305 | End: 1432 |
| Size | 20 | 20 | 280 |
(T)10c(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGCAAGCTTTGTGACAGC | ATCTGTGTGGTTGCCAGCA | Start: 956 |
| Tm | 59.335 | 59.849 | End: 1171 |
| Size | 20 | 19 | 216 |
| Primer 2 details |
|---|
| Sequence | TCTGCAAGCTTTGTGACAGC | CATCTGTGTGGTTGCCAGCA | Start: 956 |
| Tm | 59.335 | 61.174 | End: 1172 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
|---|
| Sequence | TCTGCAAGCTTTGTGACAGC | ACATCTGTGTGGTTGCCAGC | Start: 956 |
| Tm | 59.335 | 61.175 | End: 1173 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
|---|
| Sequence | TGCTGGCAACCACACAGAT | TGGAGATGCTCAAGCCAACT | Start: 1153 |
| Tm | 59.849 | 59.305 | End: 1432 |
| Size | 19 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | TGCTGGCAACCACACAGATG | TGGAGATGCTCAAGCCAACT | Start: 1153 |
| Tm | 61.174 | 59.305 | End: 1432 |
| Size | 20 | 20 | 280 |
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