ssr_primer
Primer Details for NODE_195583_length_1399_cov_136.401207 |
|---|
| (TGAT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGGCCCATCCACTAGTTA | ATTGCACTCGAGGAGCCATC | Start: 660 |
| Tm | 59.955 | 60.179 | End: 853 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TTGGTGTCTTCCCTTGCCAT | TAACTAGTGGATGGGCCCCA | Start: 574 |
| Tm | 59.518 | 59.955 | End: 679 |
| Size | 20 | 20 | 106 |
| Primer 3 details |
|---|
| Sequence | ATTGGTGTCTTCCCTTGCCA | TAACTAGTGGATGGGCCCCA | Start: 573 |
| Tm | 59.518 | 59.955 | End: 679 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | TTGACTGTCACCGTGACCAA | TAACTAGTGGATGGGCCCCA | Start: 477 |
| Tm | 59.462 | 59.955 | End: 679 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | TTTGACTGTCACCGTGACCA | TAACTAGTGGATGGGCCCCA | Start: 476 |
| Tm | 59.462 | 59.955 | End: 679 |
| Size | 20 | 20 | 204 |
(A)10taaaataacttaaaataactgtcaaaactggtcaaaactg(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGGCCCATCCACTAGTTA | ATTGCACTCGAGGAGCCATC | Start: 660 |
| Tm | 59.955 | 60.179 | End: 853 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TTGGTGTCTTCCCTTGCCAT | TAACTAGTGGATGGGCCCCA | Start: 574 |
| Tm | 59.518 | 59.955 | End: 679 |
| Size | 20 | 20 | 106 |
| Primer 3 details |
|---|
| Sequence | ATTGGTGTCTTCCCTTGCCA | TAACTAGTGGATGGGCCCCA | Start: 573 |
| Tm | 59.518 | 59.955 | End: 679 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | TTGACTGTCACCGTGACCAA | TAACTAGTGGATGGGCCCCA | Start: 477 |
| Tm | 59.462 | 59.955 | End: 679 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | TTTGACTGTCACCGTGACCA | TAACTAGTGGATGGGCCCCA | Start: 476 |
| Tm | 59.462 | 59.955 | End: 679 |
| Size | 20 | 20 | 204 |
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