ssr_primer
Primer Details for NODE_195386_length_482_cov_125.628362 |
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| (A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGCACAAAACAAACACGGA | TGTTTGCACTGAGGGAGATTCT | Start: 71 |
| Tm | 59.458 | 59.627 | End: 338 |
| Size | 21 | 22 | 268 |
| Primer 2 details |
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| Sequence | CAGCCCCTTAGCTTTCCTCA | AGGTTCCAAAGAATCACCTGTCT | Start: 7 |
| Tm | 59.379 | 59.604 | End: 172 |
| Size | 20 | 23 | 166 |
| Primer 3 details |
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| Sequence | GCAGCCCCTTAGCTTTCCTC | AGGTTCCAAAGAATCACCTGTCT | Start: 6 |
| Tm | 60.75 | 59.604 | End: 172 |
| Size | 20 | 23 | 167 |
| Primer 4 details |
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| Sequence | GAGCAGCCCCTTAGCTTTCC | AGGTTCCAAAGAATCACCTGTCT | Start: 4 |
| Tm | 60.75 | 59.604 | End: 172 |
| Size | 20 | 23 | 169 |
| Primer 5 details |
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| Sequence | GGAGCAGCCCCTTAGCTTTC | AGGTTCCAAAGAATCACCTGTCT | Start: 3 |
| Tm | 60.75 | 59.604 | End: 172 |
| Size | 20 | 23 | 170 |
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