ssr_primer
Primer Details for NODE_195090_length_1449_cov_132.665698 |
|---|
| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCCATGCTGTGTTCGTGT | TCACCAAGGAACGGACACAA | Start: 914 |
| Tm | 60.179 | 59.458 | End: 1026 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
|---|
| Sequence | TTCCCATGCTGTGTTCGTGT | ACCAAGGAACGGACACAAGT | Start: 914 |
| Tm | 60.179 | 59.455 | End: 1024 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | TTCCCATGCTGTGTTCGTGT | ATCACCAAGGAACGGACACA | Start: 914 |
| Tm | 60.179 | 59.238 | End: 1027 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | TGTGCGATTGTAAAGTGCGC | TACTGGCACACGAACACAGC | Start: 720 |
| Tm | 60.11 | 60.879 | End: 940 |
| Size | 20 | 20 | 221 |
| Primer 5 details |
|---|
| Sequence | TTCCCATGCTGTGTTCGTGT | AGGAACGGACACAAGTCACA | Start: 914 |
| Tm | 60.179 | 59.172 | End: 1020 |
| Size | 20 | 20 | 107 |
(A)12taaagtaggataaagtaggacttgaaataacttgaaat(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCCATGCTGTGTTCGTGT | TCACCAAGGAACGGACACAA | Start: 914 |
| Tm | 60.179 | 59.458 | End: 1026 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
|---|
| Sequence | TTCCCATGCTGTGTTCGTGT | ACCAAGGAACGGACACAAGT | Start: 914 |
| Tm | 60.179 | 59.455 | End: 1024 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | TTCCCATGCTGTGTTCGTGT | ATCACCAAGGAACGGACACA | Start: 914 |
| Tm | 60.179 | 59.238 | End: 1027 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | TGTGCGATTGTAAAGTGCGC | TACTGGCACACGAACACAGC | Start: 720 |
| Tm | 60.11 | 60.879 | End: 940 |
| Size | 20 | 20 | 221 |
| Primer 5 details |
|---|
| Sequence | TTCCCATGCTGTGTTCGTGT | AGGAACGGACACAAGTCACA | Start: 914 |
| Tm | 60.179 | 59.172 | End: 1020 |
| Size | 20 | 20 | 107 |
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