Primer Details for NODE_192956_length_216_cov_320.874126 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCATCTATTAATTGTATTGTAGCA | ACCTCAGAGGGAAGTATGCT | Start: 5 |
| Tm | 57.083 | 57.441 | End: 132 |
| Size | 26 | 20 | 128 |
| Primer 2 details |
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| Sequence | TGCCATCTATTAATTGTATTGTAGCA | TCAGAGGGAAGTATGCTAGTGC | Start: 5 |
| Tm | 57.083 | 59.304 | End: 129 |
| Size | 26 | 22 | 125 |
| Primer 3 details |
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| Sequence | TGCCATCTATTAATTGTATTGTAGCA | CAGAGGGAAGTATGCTAGTGCT | Start: 5 |
| Tm | 57.083 | 59.302 | End: 128 |
| Size | 26 | 22 | 124 |
| Primer 4 details |
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| Sequence | TGCCATCTATTAATTGTATTGTAGCA | AACCTCAGAGGGAAGTATGCT | Start: 5 |
| Tm | 57.083 | 58.161 | End: 133 |
| Size | 26 | 21 | 129 |
| Primer 5 details |
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| Sequence | TGCCATCTATTAATTGTATTGTAGCA | AGAGGGAAGTATGCTAGTGCT | Start: 5 |
| Tm | 57.083 | 57.961 | End: 127 |
| Size | 26 | 21 | 123 |
