ssr_primer
Primer Details for NODE_187129_length_1355_cov_75.560842 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCCGCAGAGCAAAACATG | CCAAGTGGATCGAGTGGACC | Start: 403 |
| Tm | 59.83 | 60.108 | End: 655 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
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| Sequence | ACCGGCATTCACTCACTAGC | CATGTTTTGCTCTGCGGGAG | Start: 294 |
| Tm | 60.108 | 59.83 | End: 422 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
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| Sequence | CCATACGTAAAGGCGAGCCA | CCAAGTGGATCGAGTGGACC | Start: 451 |
| Tm | 60.179 | 60.108 | End: 655 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
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| Sequence | ACCGGCATTCACTCACTAGC | TGGCTCGCCTTTACGTATGG | Start: 294 |
| Tm | 60.108 | 60.179 | End: 470 |
| Size | 20 | 20 | 177 |
| Primer 5 details |
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| Sequence | TGTGGGGAATCGCATGCATA | GCTAGTGAGTGAATGCCGGT | Start: 145 |
| Tm | 59.818 | 60.108 | End: 313 |
| Size | 20 | 20 | 169 |
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