ssr_primer
Primer Details for NODE_186032_length_949_cov_38.434932 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGAGCACTTGGTCACTAGCA | ACTAGCCCATCAGCAGTTCG | Start: 646 |
| Tm | 59.999 | 59.823 | End: 887 |
| Size | 21 | 20 | 242 |
| Primer 2 details |
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| Sequence | TGAGCACTTGGTCACTAGCA | ACTAGCCCATCAGCAGTTCG | Start: 647 |
| Tm | 58.952 | 59.823 | End: 887 |
| Size | 20 | 20 | 241 |
| Primer 3 details |
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| Sequence | TGCGCCTAAAACAAGGTCGA | TGCTAGTGACCAAGTGCTCAG | Start: 452 |
| Tm | 60.25 | 59.999 | End: 666 |
| Size | 20 | 21 | 215 |
| Primer 4 details |
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| Sequence | TGCGCCTAAAACAAGGTCGA | TGCTAGTGACCAAGTGCTCA | Start: 452 |
| Tm | 60.25 | 58.952 | End: 666 |
| Size | 20 | 20 | 215 |
| Primer 5 details |
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| Sequence | TGTGAACTGAAGGGCCAGAA | TCTGAAGGGAAGGAGTGGGA | Start: 1 |
| Tm | 59.157 | 59.508 | End: 181 |
| Size | 20 | 20 | 181 |
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