Primer Details for NODE_185434_length_263_cov_17.126316 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTAACTTGTGCCGCCTCAA | TCACGATTTATTTCACAAGGTCCA | Start: 75 |
| Tm | 59.892 | 58.69 | End: 183 |
| Size | 20 | 24 | 109 |
| Primer 2 details | |||
| Sequence | ACTAACTTGTGCCGCCTCAA | CACGATTTATTTCACAAGGTCCA | Start: 75 |
| Tm | 59.892 | 57.379 | End: 182 |
| Size | 20 | 23 | 108 |
| Primer 3 details | |||
| Sequence | ACTAACTTGTGCCGCCTCAA | TCACGATTTATTTCACAAGGTCC | Start: 75 |
| Tm | 59.892 | 57.127 | End: 183 |
| Size | 20 | 23 | 109 |
| Primer 4 details | |||
| Sequence | ACTAACTTGTGCCGCCTCAA | TCACGATTTATTTCACAAGGTCCAT | Start: 75 |
| Tm | 59.892 | 59.057 | End: 183 |
| Size | 20 | 25 | 109 |
| Primer 5 details | |||
| Sequence | ACTAACTTGTGCCGCCTCAA | ATCACGATTTATTTCACAAGGTCCA | Start: 75 |
| Tm | 59.892 | 59.057 | End: 184 |
| Size | 20 | 25 | 110 |
