ssr_primer
Primer Details for NODE_185170_length_2678_cov_47.241843 |
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| (TGA)5cgatgacgacgacgcagttggtggagcacaa(GAT)6gaggaagaggatggtcgcaaacctct(TGA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGTAGGAGGGCCAGCCTTA | ATTGCTGGCACATTGCTTGG | Start: 149 |
| Tm | 59.956 | 60.037 | End: 338 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
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| Sequence | GATCAAGTAGGAGGGCCAGC | ATTGCTGGCACATTGCTTGG | Start: 145 |
| Tm | 59.892 | 60.037 | End: 338 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
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| Sequence | TGCAAGCAAACATGGTCTGC | GCTGCAGACTGAGTAGAGGC | Start: 1619 |
| Tm | 59.968 | 60.179 | End: 1890 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
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| Sequence | GCCTCTACTCAGTCTGCAGC | GCACTTCCGAGGACAAGTCA | Start: 1871 |
| Tm | 60.179 | 59.966 | End: 2084 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | GCCTCTACTCAGTCTGCAGC | ATAGCACAAACAGGACGCGA | Start: 1871 |
| Tm | 60.179 | 60.038 | End: 2020 |
| Size | 20 | 20 | 150 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGTAGGAGGGCCAGCCTTA | ATTGCTGGCACATTGCTTGG | Start: 149 |
| Tm | 59.956 | 60.037 | End: 338 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | GATCAAGTAGGAGGGCCAGC | ATTGCTGGCACATTGCTTGG | Start: 145 |
| Tm | 59.892 | 60.037 | End: 338 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
|---|
| Sequence | TGCAAGCAAACATGGTCTGC | GCTGCAGACTGAGTAGAGGC | Start: 1619 |
| Tm | 59.968 | 60.179 | End: 1890 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | GCCTCTACTCAGTCTGCAGC | GCACTTCCGAGGACAAGTCA | Start: 1871 |
| Tm | 60.179 | 59.966 | End: 2084 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | GCCTCTACTCAGTCTGCAGC | ATAGCACAAACAGGACGCGA | Start: 1871 |
| Tm | 60.179 | 60.038 | End: 2020 |
| Size | 20 | 20 | 150 |
(A)11taaaggagaataaaggagagccacagaacgccacagaac(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGTAGGAGGGCCAGCCTTA | ATTGCTGGCACATTGCTTGG | Start: 149 |
| Tm | 59.956 | 60.037 | End: 338 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | GATCAAGTAGGAGGGCCAGC | ATTGCTGGCACATTGCTTGG | Start: 145 |
| Tm | 59.892 | 60.037 | End: 338 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
|---|
| Sequence | TGCAAGCAAACATGGTCTGC | GCTGCAGACTGAGTAGAGGC | Start: 1619 |
| Tm | 59.968 | 60.179 | End: 1890 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | GCCTCTACTCAGTCTGCAGC | GCACTTCCGAGGACAAGTCA | Start: 1871 |
| Tm | 60.179 | 59.966 | End: 2084 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | GCCTCTACTCAGTCTGCAGC | ATAGCACAAACAGGACGCGA | Start: 1871 |
| Tm | 60.179 | 60.038 | End: 2020 |
| Size | 20 | 20 | 150 |
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