Primer Details for NODE_185057_length_346_cov_312.124542 | |||
|---|---|---|---|
| (A)21: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATGACGAGCCTGCACTACT | ACTCATCTGCATTCTCTGTCTT | Start: 155 |
| Tm | 59.102 | 57.177 | End: 344 |
| Size | 20 | 22 | 190 |
| Primer 2 details | |||
| Sequence | AATGACGAGCCTGCACTACT | AACTCATCTGCATTCTCTGTCT | Start: 155 |
| Tm | 59.102 | 57.177 | End: 345 |
| Size | 20 | 22 | 191 |
| Primer 3 details | |||
| Sequence | TGGTAGTAAGAAAGCTCTGTTTCGA | AGTAGTGCAGGCTCGTCATT | Start: 45 |
| Tm | 59.99 | 59.102 | End: 174 |
| Size | 25 | 20 | 130 |
| Primer 4 details | |||
| Sequence | AATGACGAGCCTGCACTACT | ACTCATCTGCATTCTCTGTCTTT | Start: 155 |
| Tm | 59.102 | 57.837 | End: 344 |
| Size | 20 | 23 | 190 |
| Primer 5 details | |||
| Sequence | AATGACGAGCCTGCACTACT | AACTCATCTGCATTCTCTGTCTT | Start: 155 |
| Tm | 59.102 | 57.837 | End: 345 |
| Size | 20 | 23 | 191 |
