ssr_primer
Primer Details for NODE_183887_length_879_cov_62.074442 |
|---|
| (T)19: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGAGGTACCTGTAGCTGTCT | ACTGCACCAAGAGATCACAGA | Start: 188 |
| Tm | 59.995 | 59.026 | End: 325 |
| Size | 21 | 21 | 138 |
| Primer 2 details |
|---|
| Sequence | ACGAGGTACCTGTAGCTGTCT | ACTGCACCAAGAGATCACAGAG | Start: 188 |
| Tm | 59.995 | 60.028 | End: 325 |
| Size | 21 | 22 | 138 |
| Primer 3 details |
|---|
| Sequence | TCTGTGATCTCTTGGTGCAGT | ACCGCCATCTCATGAGAACT | Start: 305 |
| Tm | 59.026 | 58.801 | End: 531 |
| Size | 21 | 20 | 227 |
| Primer 4 details |
|---|
| Sequence | CTCTGTGATCTCTTGGTGCAGT | ACCGCCATCTCATGAGAACT | Start: 304 |
| Tm | 60.028 | 58.801 | End: 531 |
| Size | 22 | 20 | 228 |
| Primer 5 details |
|---|
| Sequence | ACGAGGTACCTGTAGCTGTCT | CTGCACCAAGAGATCACAGAGA | Start: 188 |
| Tm | 59.995 | 59.767 | End: 324 |
| Size | 21 | 22 | 137 |
(A)10cactgtacatcactgtacatcagcacgatgcagcacgatg(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGAGGTACCTGTAGCTGTCT | ACTGCACCAAGAGATCACAGA | Start: 188 |
| Tm | 59.995 | 59.026 | End: 325 |
| Size | 21 | 21 | 138 |
| Primer 2 details |
|---|
| Sequence | ACGAGGTACCTGTAGCTGTCT | ACTGCACCAAGAGATCACAGAG | Start: 188 |
| Tm | 59.995 | 60.028 | End: 325 |
| Size | 21 | 22 | 138 |
| Primer 3 details |
|---|
| Sequence | TCTGTGATCTCTTGGTGCAGT | ACCGCCATCTCATGAGAACT | Start: 305 |
| Tm | 59.026 | 58.801 | End: 531 |
| Size | 21 | 20 | 227 |
| Primer 4 details |
|---|
| Sequence | CTCTGTGATCTCTTGGTGCAGT | ACCGCCATCTCATGAGAACT | Start: 304 |
| Tm | 60.028 | 58.801 | End: 531 |
| Size | 22 | 20 | 228 |
| Primer 5 details |
|---|
| Sequence | ACGAGGTACCTGTAGCTGTCT | CTGCACCAAGAGATCACAGAGA | Start: 188 |
| Tm | 59.995 | 59.767 | End: 324 |
| Size | 21 | 22 | 137 |
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