ssr_primer
Primer Details for NODE_183551_length_3277_cov_40.572409 |
|---|
| (T)10cggttttccgcggttttccgaactttatttaacttta(T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAACAAACATGGGCCGAT | TATACAACCGTCTCGCTCGC | Start: 1036 |
| Tm | 60.037 | 59.971 | End: 1283 |
| Size | 20 | 20 | 248 |
| Primer 2 details |
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| Sequence | GGCGGGTGAAAGGTACAAGA | GACTGAGTCAACAGAGGCCC | Start: 508 |
| Tm | 59.964 | 60.037 | End: 759 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
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| Sequence | TACAAGGCGGTATGTGCGTT | GACTGAGTCAACAGAGGCCC | Start: 528 |
| Tm | 60.037 | 60.037 | End: 759 |
| Size | 20 | 20 | 232 |
| Primer 4 details |
|---|
| Sequence | GGCGGGTGAAAGGTACAAGA | AAGGGAAAGGGGCCTAAAGC | Start: 508 |
| Tm | 59.964 | 59.959 | End: 724 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | TACAAGGCGGTATGTGCGTT | AAGGGAAAGGGGCCTAAAGC | Start: 528 |
| Tm | 60.037 | 59.959 | End: 724 |
| Size | 20 | 20 | 197 |
(AAC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAACAAACATGGGCCGAT | TATACAACCGTCTCGCTCGC | Start: 1036 |
| Tm | 60.037 | 59.971 | End: 1283 |
| Size | 20 | 20 | 248 |
| Primer 2 details |
|---|
| Sequence | GGCGGGTGAAAGGTACAAGA | GACTGAGTCAACAGAGGCCC | Start: 508 |
| Tm | 59.964 | 60.037 | End: 759 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | TACAAGGCGGTATGTGCGTT | GACTGAGTCAACAGAGGCCC | Start: 528 |
| Tm | 60.037 | 60.037 | End: 759 |
| Size | 20 | 20 | 232 |
| Primer 4 details |
|---|
| Sequence | GGCGGGTGAAAGGTACAAGA | AAGGGAAAGGGGCCTAAAGC | Start: 508 |
| Tm | 59.964 | 59.959 | End: 724 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | TACAAGGCGGTATGTGCGTT | AAGGGAAAGGGGCCTAAAGC | Start: 528 |
| Tm | 60.037 | 59.959 | End: 724 |
| Size | 20 | 20 | 197 |
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