Primer Details for NODE_182353_length_248_cov_10.571429 | |||
|---|---|---|---|
| (A)16: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTCGATGAATTTATCTTCACCCT | TGCTCTTGATCTTCTGCTCACT | Start: 106 |
| Tm | 60.105 | 59.434 | End: 222 |
| Size | 25 | 22 | 117 |
| Primer 2 details | |||
| Sequence | TGGTCGATGAATTTATCTTCACCC | TGCTCTTGATCTTCTGCTCACT | Start: 106 |
| Tm | 58.877 | 59.434 | End: 222 |
| Size | 24 | 22 | 117 |
| Primer 3 details | |||
| Sequence | TGGTCGATGAATTTATCTTCACCCT | TCTGCTCTTGATCTTCTGCTCA | Start: 106 |
| Tm | 60.105 | 59.17 | End: 224 |
| Size | 25 | 22 | 119 |
| Primer 4 details | |||
| Sequence | GGTCGATGAATTTATCTTCACCCT | TGCTCTTGATCTTCTGCTCACT | Start: 107 |
| Tm | 58.631 | 59.434 | End: 222 |
| Size | 24 | 22 | 116 |
| Primer 5 details | |||
| Sequence | TGGTCGATGAATTTATCTTCACCC | TCTGCTCTTGATCTTCTGCTCA | Start: 106 |
| Tm | 58.877 | 59.17 | End: 224 |
| Size | 24 | 22 | 119 |
