ssr_primer
Primer Details for NODE_182234_length_1224_cov_20.434405 |
|---|
| (CGAGC)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCCATGTTGAACTGCACC | CTGCGAGCAACACGATGATG | Start: 847 |
| Tm | 60.039 | 59.974 | End: 1107 |
| Size | 20 | 20 | 261 |
| Primer 2 details |
|---|
| Sequence | CCTGCCATGTTGAACTGCAC | CTGCGAGCAACACGATGATG | Start: 846 |
| Tm | 60.039 | 59.974 | End: 1107 |
| Size | 20 | 20 | 262 |
| Primer 3 details |
|---|
| Sequence | TCGTGTTGCTCGCAGTTACT | AGCAACACGATGATGAGGCA | Start: 1093 |
| Tm | 59.968 | 60.036 | End: 1212 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
|---|
| Sequence | TCGTGTTGCTCGCAGTTACT | ACACGGAGCAACACGATGAT | Start: 1093 |
| Tm | 59.968 | 60.037 | End: 1218 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | CTGATTGCCATGGTCCTGGT | AGCAACACGATGATGAGGCA | Start: 1062 |
| Tm | 60.034 | 60.036 | End: 1212 |
| Size | 20 | 20 | 151 |
(GT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCCATGTTGAACTGCACC | CTGCGAGCAACACGATGATG | Start: 847 |
| Tm | 60.039 | 59.974 | End: 1107 |
| Size | 20 | 20 | 261 |
| Primer 2 details |
|---|
| Sequence | CCTGCCATGTTGAACTGCAC | CTGCGAGCAACACGATGATG | Start: 846 |
| Tm | 60.039 | 59.974 | End: 1107 |
| Size | 20 | 20 | 262 |
| Primer 3 details |
|---|
| Sequence | TCGTGTTGCTCGCAGTTACT | AGCAACACGATGATGAGGCA | Start: 1093 |
| Tm | 59.968 | 60.036 | End: 1212 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
|---|
| Sequence | TCGTGTTGCTCGCAGTTACT | ACACGGAGCAACACGATGAT | Start: 1093 |
| Tm | 59.968 | 60.037 | End: 1218 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | CTGATTGCCATGGTCCTGGT | AGCAACACGATGATGAGGCA | Start: 1062 |
| Tm | 60.034 | 60.036 | End: 1212 |
| Size | 20 | 20 | 151 |
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