ssr_primer
Primer Details for NODE_174781_length_748_cov_3.754074 |
|---|
| (G)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTCCTCACCTCCATCCTA | GGACGCACCCTACTGATAGC | Start: 379 |
| Tm | 60.105 | 59.968 | End: 554 |
| Size | 20 | 20 | 176 |
| Primer 2 details |
|---|
| Sequence | GCCTCCTCACCTCCATCCTA | ACGTAGCAACAGCCCTCTTC | Start: 379 |
| Tm | 60.105 | 60.037 | End: 608 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
|---|
| Sequence | GCCTCCTCACCTCCATCCTA | GACGTAGCAACAGCCCTCTT | Start: 379 |
| Tm | 60.105 | 60.037 | End: 609 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | GCTATCAGTAGGGTGCGTCC | ACCTTGATGTCCTCCAGGGA | Start: 535 |
| Tm | 59.968 | 59.881 | End: 660 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | ATTCCAGCTTCGCCTACACC | TAGGATGGAGGTGAGGAGGC | Start: 138 |
| Tm | 60.108 | 60.105 | End: 398 |
| Size | 20 | 20 | 261 |
(TGT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTCCTCACCTCCATCCTA | GGACGCACCCTACTGATAGC | Start: 379 |
| Tm | 60.105 | 59.968 | End: 554 |
| Size | 20 | 20 | 176 |
| Primer 2 details |
|---|
| Sequence | GCCTCCTCACCTCCATCCTA | ACGTAGCAACAGCCCTCTTC | Start: 379 |
| Tm | 60.105 | 60.037 | End: 608 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
|---|
| Sequence | GCCTCCTCACCTCCATCCTA | GACGTAGCAACAGCCCTCTT | Start: 379 |
| Tm | 60.105 | 60.037 | End: 609 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | GCTATCAGTAGGGTGCGTCC | ACCTTGATGTCCTCCAGGGA | Start: 535 |
| Tm | 59.968 | 59.881 | End: 660 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | ATTCCAGCTTCGCCTACACC | TAGGATGGAGGTGAGGAGGC | Start: 138 |
| Tm | 60.108 | 60.105 | End: 398 |
| Size | 20 | 20 | 261 |
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