ssr_primer
Primer Details for NODE_173381_length_2570_cov_52.167801 |
|---|
| (A)10tcatttaagt(A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCCTTGGTCTTGTTCTCCA | GACTCTCCTCAGATGACGCG | Start: 1229 |
| Tm | 59.89 | 59.971 | End: 1431 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | GGCTAGCCAAGTTGCTACCA | CCTTCACAAGTGGGCAAGGA | Start: 1989 |
| Tm | 60.035 | 60.179 | End: 2100 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | GGCTAGCCAAGTTGCTACCA | TCCTTCACAAGTGGGCAAGG | Start: 1989 |
| Tm | 60.035 | 60.179 | End: 2101 |
| Size | 20 | 20 | 113 |
| Primer 4 details |
|---|
| Sequence | CAGTCAACCTAGACCTGCGG | TGGAGAACAAGACCAAGGCC | Start: 1094 |
| Tm | 60.109 | 59.89 | End: 1248 |
| Size | 20 | 20 | 155 |
| Primer 5 details |
|---|
| Sequence | GCGGGACAGCAGAGTCATAA | TGGAGAACAAGACCAAGGCC | Start: 1110 |
| Tm | 59.824 | 59.89 | End: 1248 |
| Size | 20 | 20 | 139 |
(A)22ttctgagcgttga(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCCTTGGTCTTGTTCTCCA | GACTCTCCTCAGATGACGCG | Start: 1229 |
| Tm | 59.89 | 59.971 | End: 1431 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | GGCTAGCCAAGTTGCTACCA | CCTTCACAAGTGGGCAAGGA | Start: 1989 |
| Tm | 60.035 | 60.179 | End: 2100 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | GGCTAGCCAAGTTGCTACCA | TCCTTCACAAGTGGGCAAGG | Start: 1989 |
| Tm | 60.035 | 60.179 | End: 2101 |
| Size | 20 | 20 | 113 |
| Primer 4 details |
|---|
| Sequence | CAGTCAACCTAGACCTGCGG | TGGAGAACAAGACCAAGGCC | Start: 1094 |
| Tm | 60.109 | 59.89 | End: 1248 |
| Size | 20 | 20 | 155 |
| Primer 5 details |
|---|
| Sequence | GCGGGACAGCAGAGTCATAA | TGGAGAACAAGACCAAGGCC | Start: 1110 |
| Tm | 59.824 | 59.89 | End: 1248 |
| Size | 20 | 20 | 139 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCCTTGGTCTTGTTCTCCA | GACTCTCCTCAGATGACGCG | Start: 1229 |
| Tm | 59.89 | 59.971 | End: 1431 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | GGCTAGCCAAGTTGCTACCA | CCTTCACAAGTGGGCAAGGA | Start: 1989 |
| Tm | 60.035 | 60.179 | End: 2100 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | GGCTAGCCAAGTTGCTACCA | TCCTTCACAAGTGGGCAAGG | Start: 1989 |
| Tm | 60.035 | 60.179 | End: 2101 |
| Size | 20 | 20 | 113 |
| Primer 4 details |
|---|
| Sequence | CAGTCAACCTAGACCTGCGG | TGGAGAACAAGACCAAGGCC | Start: 1094 |
| Tm | 60.109 | 59.89 | End: 1248 |
| Size | 20 | 20 | 155 |
| Primer 5 details |
|---|
| Sequence | GCGGGACAGCAGAGTCATAA | TGGAGAACAAGACCAAGGCC | Start: 1110 |
| Tm | 59.824 | 59.89 | End: 1248 |
| Size | 20 | 20 | 139 |
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