ssr_primer
Primer Details for NODE_169415_length_1064_cov_21.771948 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCACGTTGGTTAGCGTATG | CTGGAGCTCAGCCAACATGA | Start: 225 |
| Tm | 59.976 | 60.036 | End: 465 |
| Size | 20 | 20 | 241 |
| Primer 2 details |
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| Sequence | TTTGTGCGCACGTTGGTTAG | CTGGAGCTCAGCCAACATGA | Start: 219 |
| Tm | 59.972 | 60.036 | End: 465 |
| Size | 20 | 20 | 247 |
| Primer 3 details |
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| Sequence | TGCTGCGTCCAGAAAAGGAT | CTAACCAACGTGCGCACAAA | Start: 78 |
| Tm | 59.963 | 59.972 | End: 238 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
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| Sequence | CAACAACAAGCGATGGGGTG | CTAACCAACGTGCGCACAAA | Start: 55 |
| Tm | 60.039 | 59.972 | End: 238 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
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| Sequence | GTTGCTGCGTCCAGAAAAGG | CTAACCAACGTGCGCACAAA | Start: 76 |
| Tm | 60.04 | 59.972 | End: 238 |
| Size | 20 | 20 | 163 |
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