ssr_primer
Primer Details for NODE_168915_length_2151_cov_87.501444 |
|---|
| (TC)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCATTGGACGTGTCTCTGT | AGCAAGAGAGAGCCAGAGAGA | Start: 60 |
| Tm | 59.607 | 59.994 | End: 335 |
| Size | 20 | 21 | 276 |
| Primer 2 details |
|---|
| Sequence | ATTTGCTTTGGAAGGCAGGC | ATGGTGTCTGGAGAGGAGTGA | Start: 1662 |
| Tm | 59.677 | 59.922 | End: 1761 |
| Size | 20 | 21 | 100 |
| Primer 3 details |
|---|
| Sequence | CCCTCTCTCTCCCTCTCTGT | TCACCTGGGACCAGAAAACC | Start: 449 |
| Tm | 59.074 | 59.523 | End: 647 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | CCCTCTCTCTCCCTCTCTGT | CACCTGGGACCAGAAAACCT | Start: 449 |
| Tm | 59.074 | 59.521 | End: 646 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | TCTCCCTCTCTCTCCCTCTCT | TCACCTGGGACCAGAAAACC | Start: 446 |
| Tm | 59.989 | 59.523 | End: 647 |
| Size | 21 | 20 | 202 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCATTGGACGTGTCTCTGT | AGCAAGAGAGAGCCAGAGAGA | Start: 60 |
| Tm | 59.607 | 59.994 | End: 335 |
| Size | 20 | 21 | 276 |
| Primer 2 details |
|---|
| Sequence | ATTTGCTTTGGAAGGCAGGC | ATGGTGTCTGGAGAGGAGTGA | Start: 1662 |
| Tm | 59.677 | 59.922 | End: 1761 |
| Size | 20 | 21 | 100 |
| Primer 3 details |
|---|
| Sequence | CCCTCTCTCTCCCTCTCTGT | TCACCTGGGACCAGAAAACC | Start: 449 |
| Tm | 59.074 | 59.523 | End: 647 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | CCCTCTCTCTCCCTCTCTGT | CACCTGGGACCAGAAAACCT | Start: 449 |
| Tm | 59.074 | 59.521 | End: 646 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | TCTCCCTCTCTCTCCCTCTCT | TCACCTGGGACCAGAAAACC | Start: 446 |
| Tm | 59.989 | 59.523 | End: 647 |
| Size | 21 | 20 | 202 |
(TG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCATTGGACGTGTCTCTGT | AGCAAGAGAGAGCCAGAGAGA | Start: 60 |
| Tm | 59.607 | 59.994 | End: 335 |
| Size | 20 | 21 | 276 |
| Primer 2 details |
|---|
| Sequence | ATTTGCTTTGGAAGGCAGGC | ATGGTGTCTGGAGAGGAGTGA | Start: 1662 |
| Tm | 59.677 | 59.922 | End: 1761 |
| Size | 20 | 21 | 100 |
| Primer 3 details |
|---|
| Sequence | CCCTCTCTCTCCCTCTCTGT | TCACCTGGGACCAGAAAACC | Start: 449 |
| Tm | 59.074 | 59.523 | End: 647 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | CCCTCTCTCTCCCTCTCTGT | CACCTGGGACCAGAAAACCT | Start: 449 |
| Tm | 59.074 | 59.521 | End: 646 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | TCTCCCTCTCTCTCCCTCTCT | TCACCTGGGACCAGAAAACC | Start: 446 |
| Tm | 59.989 | 59.523 | End: 647 |
| Size | 21 | 20 | 202 |
(A)12taaaattttttaaaatttttaattaatcaaaattaatc(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCATTGGACGTGTCTCTGT | AGCAAGAGAGAGCCAGAGAGA | Start: 60 |
| Tm | 59.607 | 59.994 | End: 335 |
| Size | 20 | 21 | 276 |
| Primer 2 details |
|---|
| Sequence | ATTTGCTTTGGAAGGCAGGC | ATGGTGTCTGGAGAGGAGTGA | Start: 1662 |
| Tm | 59.677 | 59.922 | End: 1761 |
| Size | 20 | 21 | 100 |
| Primer 3 details |
|---|
| Sequence | CCCTCTCTCTCCCTCTCTGT | TCACCTGGGACCAGAAAACC | Start: 449 |
| Tm | 59.074 | 59.523 | End: 647 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | CCCTCTCTCTCCCTCTCTGT | CACCTGGGACCAGAAAACCT | Start: 449 |
| Tm | 59.074 | 59.521 | End: 646 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | TCTCCCTCTCTCTCCCTCTCT | TCACCTGGGACCAGAAAACC | Start: 446 |
| Tm | 59.989 | 59.523 | End: 647 |
| Size | 21 | 20 | 202 |
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