Primer Details for NODE_16753_length_213_cov_725.421429 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAACTCGGCAAATAAAATTATCCG | TCACTGAGCAGGCTGTACTTC | Start: 4 |
| Tm | 59.087 | 59.726 | End: 206 |
| Size | 25 | 21 | 203 |
| Primer 2 details |
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| Sequence | GGAACTCGGCAAATAAAATTATCCG | CACTGAGCAGGCTGTACTTCT | Start: 4 |
| Tm | 59.087 | 59.725 | End: 205 |
| Size | 25 | 21 | 202 |
| Primer 3 details |
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| Sequence | GGAACTCGGCAAATAAAATTATCCG | TCACTGAGCAGGCTGTACTT | Start: 4 |
| Tm | 59.087 | 58.658 | End: 206 |
| Size | 25 | 20 | 203 |
| Primer 4 details |
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| Sequence | GGAACTCGGCAAATAAAATTATCCG | TTCACTGAGCAGGCTGTACT | Start: 4 |
| Tm | 59.087 | 58.658 | End: 207 |
| Size | 25 | 20 | 204 |
| Primer 5 details |
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| Sequence | GGAACTCGGCAAATAAAATTATCCGA | TCACTGAGCAGGCTGTACTTC | Start: 4 |
| Tm | 60.239 | 59.726 | End: 206 |
| Size | 26 | 21 | 203 |
