ssr_primer
Primer Details for NODE_167273_length_1483_cov_21.279433 |
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| (A)11tgaatttatatgaatttatggtgtcactgggtgtcactg(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGGGAGCAGGTCTTCAAG | AGCATAATGGGGCGTGTTCA | Start: 230 |
| Tm | 60.036 | 60.035 | End: 491 |
| Size | 20 | 20 | 262 |
| Primer 2 details |
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| Sequence | CCAGGGAGCAGGTCTTCAAG | TCAGCATAATGGGGCGTGTT | Start: 230 |
| Tm | 60.036 | 60.035 | End: 493 |
| Size | 20 | 20 | 264 |
| Primer 3 details |
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| Sequence | CCTGTGTGCATGTGTGTGTG | TTTCGAATGTTGCGGCACAG | Start: 1146 |
| Tm | 59.97 | 60.041 | End: 1374 |
| Size | 20 | 20 | 229 |
| Primer 4 details |
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| Sequence | TTCTACAAGAACCCGCGCAT | CTTGAAGACCTGCTCCCTGG | Start: 133 |
| Tm | 60.037 | 60.036 | End: 249 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
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| Sequence | CCAGGGAGCAGGTCTTCAAG | TCGAAAAGCGGAGTTCCTCC | Start: 230 |
| Tm | 60.036 | 60.038 | End: 469 |
| Size | 20 | 20 | 240 |
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