ssr_primer
Primer Details for NODE_167090_length_6544_cov_24.518931 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAATGTGTCATGCCCTCCA | CTCGACTTGAGGGCAGGTTT | Start: 1134 |
| Tm | 60.034 | 59.965 | End: 1277 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
|---|
| Sequence | TGGAATGTGTCATGCCCTCC | CTCGACTTGAGGGCAGGTTT | Start: 1133 |
| Tm | 60.034 | 59.965 | End: 1277 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
|---|
| Sequence | AAACCTGCCCTCAAGTCGAG | CTGGTAAGGCTGACACTGCA | Start: 1258 |
| Tm | 59.965 | 59.965 | End: 1415 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | AAACCTGCCCTCAAGTCGAG | TGCTGGTAAGGCTGACACTG | Start: 1258 |
| Tm | 59.965 | 59.965 | End: 1417 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | CCCCAAGTTCATGCACCTCT | GCCGCTCTTGTAGGAGATCC | Start: 119 |
| Tm | 59.961 | 59.967 | End: 329 |
| Size | 20 | 20 | 211 |
(A)11ggaaagcaaaggaaagcaatttttcatgttttttcatgt(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAATGTGTCATGCCCTCCA | CTCGACTTGAGGGCAGGTTT | Start: 1134 |
| Tm | 60.034 | 59.965 | End: 1277 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
|---|
| Sequence | TGGAATGTGTCATGCCCTCC | CTCGACTTGAGGGCAGGTTT | Start: 1133 |
| Tm | 60.034 | 59.965 | End: 1277 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
|---|
| Sequence | AAACCTGCCCTCAAGTCGAG | CTGGTAAGGCTGACACTGCA | Start: 1258 |
| Tm | 59.965 | 59.965 | End: 1415 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | AAACCTGCCCTCAAGTCGAG | TGCTGGTAAGGCTGACACTG | Start: 1258 |
| Tm | 59.965 | 59.965 | End: 1417 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | CCCCAAGTTCATGCACCTCT | GCCGCTCTTGTAGGAGATCC | Start: 119 |
| Tm | 59.961 | 59.967 | End: 329 |
| Size | 20 | 20 | 211 |
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