ssr_primer
Primer Details for NODE_166881_length_1895_cov_126.572997 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGCTTGTGTCTGGTGTGT | GCAAACACGTCTACACAGTCC | Start: 357 |
| Tm | 60.036 | 59.474 | End: 529 |
| Size | 20 | 21 | 173 |
| Primer 2 details |
|---|
| Sequence | GCATACTCTCCTGTCACGCA | CACTACAGTGCAGGCCAAAT | Start: 1412 |
| Tm | 59.827 | 58.462 | End: 1685 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | TGCATACTCTCCTGTCACGC | CACTACAGTGCAGGCCAAAT | Start: 1411 |
| Tm | 59.827 | 58.462 | End: 1685 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
|---|
| Sequence | ACTGTGCTTGTGTCTGGTGT | GCAAACACGTCTACACAGTCC | Start: 355 |
| Tm | 59.747 | 59.474 | End: 529 |
| Size | 20 | 21 | 175 |
| Primer 5 details |
|---|
| Sequence | AATGTGTGGCTTCCTCGGAA | GCAAACACGTCTACACAGTCC | Start: 377 |
| Tm | 59.6 | 59.474 | End: 529 |
| Size | 20 | 21 | 153 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGCTTGTGTCTGGTGTGT | GCAAACACGTCTACACAGTCC | Start: 357 |
| Tm | 60.036 | 59.474 | End: 529 |
| Size | 20 | 21 | 173 |
| Primer 2 details |
|---|
| Sequence | GCATACTCTCCTGTCACGCA | CACTACAGTGCAGGCCAAAT | Start: 1412 |
| Tm | 59.827 | 58.462 | End: 1685 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | TGCATACTCTCCTGTCACGC | CACTACAGTGCAGGCCAAAT | Start: 1411 |
| Tm | 59.827 | 58.462 | End: 1685 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
|---|
| Sequence | ACTGTGCTTGTGTCTGGTGT | GCAAACACGTCTACACAGTCC | Start: 355 |
| Tm | 59.747 | 59.474 | End: 529 |
| Size | 20 | 21 | 175 |
| Primer 5 details |
|---|
| Sequence | AATGTGTGGCTTCCTCGGAA | GCAAACACGTCTACACAGTCC | Start: 377 |
| Tm | 59.6 | 59.474 | End: 529 |
| Size | 20 | 21 | 153 |
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