ssr_primer
Primer Details for NODE_165591_length_2931_cov_37.695591 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCGCGCTCAACTGTTTGTT | AACATCCAGGACAGGCAAGG | Start: 44 |
| Tm | 59.972 | 59.961 | End: 297 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
|---|
| Sequence | CCGTCGGGACTCCAAAAGAA | CCTCTTCAGTGAACCGCAGT | Start: 489 |
| Tm | 59.966 | 59.966 | End: 615 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | TCAAATGTCGCCCCCTAACC | CCTCTTCAGTGAACCGCAGT | Start: 469 |
| Tm | 60.035 | 59.966 | End: 615 |
| Size | 20 | 20 | 147 |
| Primer 4 details |
|---|
| Sequence | TTTCCACGGTCATGCCTCTC | CCTCTTCAGTGAACCGCAGT | Start: 400 |
| Tm | 60.036 | 59.966 | End: 615 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TTTCCACGGTCATGCCTCTC | TTCTTTTGGAGTCCCGACGG | Start: 400 |
| Tm | 60.036 | 59.966 | End: 508 |
| Size | 20 | 20 | 109 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCGCGCTCAACTGTTTGTT | AACATCCAGGACAGGCAAGG | Start: 44 |
| Tm | 59.972 | 59.961 | End: 297 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
|---|
| Sequence | CCGTCGGGACTCCAAAAGAA | CCTCTTCAGTGAACCGCAGT | Start: 489 |
| Tm | 59.966 | 59.966 | End: 615 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | TCAAATGTCGCCCCCTAACC | CCTCTTCAGTGAACCGCAGT | Start: 469 |
| Tm | 60.035 | 59.966 | End: 615 |
| Size | 20 | 20 | 147 |
| Primer 4 details |
|---|
| Sequence | TTTCCACGGTCATGCCTCTC | CCTCTTCAGTGAACCGCAGT | Start: 400 |
| Tm | 60.036 | 59.966 | End: 615 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TTTCCACGGTCATGCCTCTC | TTCTTTTGGAGTCCCGACGG | Start: 400 |
| Tm | 60.036 | 59.966 | End: 508 |
| Size | 20 | 20 | 109 |
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