ssr_primer
Primer Details for NODE_164412_length_2019_cov_135.744604 |
|---|
| (T)19: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGACCCGTACAAGTACCTC | TCTTGAAGGGACAATGGGGC | Start: 60 |
| Tm | 60.179 | 59.96 | End: 264 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | GCGACCCGTACAAGTACCTC | CCACTGGACGATGGTGAGAG | Start: 60 |
| Tm | 60.179 | 59.826 | End: 332 |
| Size | 20 | 20 | 273 |
| Primer 3 details |
|---|
| Sequence | CTCTCACCATCGTCCAGTGG | TGAGCCACACCAAAGTTCCA | Start: 313 |
| Tm | 59.826 | 59.743 | End: 588 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | AATCTGACAGGAGCAGCAGC | TCTTGAAGGGACAATGGGGC | Start: 144 |
| Tm | 60.393 | 59.96 | End: 264 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | GCCCCATTGTCCCTTCAAGA | GATTCCACCTCTGCCACTGG | Start: 245 |
| Tm | 59.96 | 60.394 | End: 345 |
| Size | 20 | 20 | 101 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGACCCGTACAAGTACCTC | TCTTGAAGGGACAATGGGGC | Start: 60 |
| Tm | 60.179 | 59.96 | End: 264 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | GCGACCCGTACAAGTACCTC | CCACTGGACGATGGTGAGAG | Start: 60 |
| Tm | 60.179 | 59.826 | End: 332 |
| Size | 20 | 20 | 273 |
| Primer 3 details |
|---|
| Sequence | CTCTCACCATCGTCCAGTGG | TGAGCCACACCAAAGTTCCA | Start: 313 |
| Tm | 59.826 | 59.743 | End: 588 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | AATCTGACAGGAGCAGCAGC | TCTTGAAGGGACAATGGGGC | Start: 144 |
| Tm | 60.393 | 59.96 | End: 264 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | GCCCCATTGTCCCTTCAAGA | GATTCCACCTCTGCCACTGG | Start: 245 |
| Tm | 59.96 | 60.394 | End: 345 |
| Size | 20 | 20 | 101 |
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